Help please on Genomic DNA extraction of CHO cells.

Mad-Woof mad-woof at ROCKETMAIL.COM
Wed Jun 23 20:10:40 EST 1999

I have this problem of genomic DNA extraction of CHO cells.

After incubated overnight at 50 degrees centigrade in the lysis
buffer, a big blob of thing is found floating in my solution. Even
after extraction with phenol and phenol:chloroform, the big blob was
still there in the aqueous phase. And the DNA yield was also very low. 
Help please!

Seah Kwee Loong
z2218244 at

Get your free address at

More information about the Methods mailing list