Help please on Genomic DNA extraction of CHO cells.

Mad-Woof mad-woof at ROCKETMAIL.COM
Wed Jun 23 20:10:40 EST 1999


I have this problem of genomic DNA extraction of CHO cells.

After incubated overnight at 50 degrees centigrade in the lysis
buffer, a big blob of thing is found floating in my solution. Even
after extraction with phenol and phenol:chloroform, the big blob was
still there in the aqueous phase. And the DNA yield was also very low. 
Help please!

Seah Kwee Loong
z2218244 at student.unsw.edu.au





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