DNA quantification

mouse jpowell at sunstroke.sdsu.edu
Thu Jun 24 01:31:35 EST 1999


George Rutherford <gruther at bilbo.bio.purdue.edu> wrote in message
news:gruther-2206991452300001 at arbpc3.btny.purdue.edu...
> In article <7kb26p$2bv$1 at testinfo.uoguelph.ca>, kurbanic at uoguelph.ca
> (Kevin Urbanic) wrote:
>
> > I would like to know if anybody has a method of determining [DNA] other
> > than using a UV-vis spectrophotometer.  Our departmental spec seems to
> > provided extremely inconsistent concentrations when I spec plasmid DNA
> > that is at a concentration less than 1ug/ml.  Specifcally, I was
> > wondering if anybody has calculated the concentration of each band in a
> > 1kb DNA ladder?  I thought that could be a useful tool for "eyeing" the
> > DNA concentration.
>
> Fluorimetry

If you're talking about EtBr stained gels then :

1 ug of 1kb lader ~ 75 ng per band





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