help

beisz at SUN5.IBP.AC.CN beisz at SUN5.IBP.AC.CN
Thu Jun 24 02:25:45 EST 1999


I have seperated RNA from the yolk of eggs, but have some problem in futher
purifying the RNA. The A260/A280 of the samples I obtained was about 1.2,
and I have tried the following ways to purify it:
1. percipitate the sample with LiCl
2. repeat the extraction with phenol(pH4.0):chloroform
3. purify the sample with Sepherose CL-6B
4. recover the RNA after eletropheresis
But all these failed to improve the value of A260/A280. So please inform me
if there is any other good suggestion.

Thanks!

Sincerely yours

LEI Haixin
Department of Cell Biology,
Institute of Biophysics,
Chinese Academy of Sciences
15 Datun Road, Chao-yang District
Beijing 100101, P R China
Tel:  0086-10-64888568
Fax: 0086-10-64877837
E-mail: <beisz at sun5.ibp.ac.cn>




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