Primers for sequencing from EMBL4?
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Thu Jun 24 03:11:44 EST 1999
In article <3770d87b.0 at moron.semo.edu>, Allen Gathman <agathman.nospam at b
>I have an old genomic library in EMBL4, and would like to sequence some
>clones from it. Unlike most newer vectors, EMBL4 doesn't have T3, T7, or
>any other standard flanking promoter to use as a sequencing primer. Does
>anyone have experience sequencing from EMBL4? Maybe someone has already
>generated some flanking primers and could send me sequences for them, so I
>can save the trouble of designing and testing new ones?
^ add a space here and most e-mail software will automatically remove
the sig. below
>agathman at biology.semo.edu
Try the following. I can't remember where I got them from, possibly this
newsgroup. The archives would tell you.
EMBL PCR Primers for Lambda
5 TGC CCG AGA AGA TGT TGA GCA AAC 3
5 TCT GGG CTT AAA AGC AGA AGT CCA AC 3
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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