Southern hybridization

Hiranya S. Roychowdhury hroychow at NMSU.EDU
Thu Jun 24 17:41:01 EST 1999

At 02:36 AM 6/24/99 -0700, Dr.Sh.Shahrul Rabiah wrote:
>My Southern is not working. No signals at all. Would a low abundance
>gene result in this. Help!!
I am having the same problem. The probe is absolutely homologous, but no
signal from the genomic DNA. I suspect very low/single copy gene. I would
like to get some input too from those that deal (or have dealt) with single
copy genes.



Dr. Hiranya Sankar Roychowdhury
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at

More information about the Methods mailing list