Southern hybridization

Hiranya S. Roychowdhury hroychow at NMSU.EDU
Thu Jun 24 17:41:01 EST 1999


At 02:36 AM 6/24/99 -0700, Dr.Sh.Shahrul Rabiah wrote:
>My Southern is not working. No signals at all. Would a low abundance
>gene result in this. Help!!
>
>Sharifah.
>
>
>
I am having the same problem. The probe is absolutely homologous, but no
signal from the genomic DNA. I suspect very low/single copy gene. I would
like to get some input too from those that deal (or have dealt) with single
copy genes.

Thanks,

Hiranya.


Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu




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