internal standard for RT-PCR in plant

Mike Prigge prigge at darkwing.uoregon.edu
Thu Jun 24 19:39:16 EST 1999


In article <7kl7ed$7n3$1 at nnrp1.deja.com>, myoon at hort.cri.nz wrote:

> Hi all,
> 
> I'm interested in RT-PCR for gene expression studies.  It would be
> appreciated if you could recommend some gene(s) or any sequence suitable
> as a internal control for arabidopsis thaliana.
> 
> Thanks in advance
> Minsoo
> 
> 
For leaves, I have used beta-ATPase (mitochondrial, I believe) and ROC1
(involved with ubiquitination but I cannot remember off hand how).  It was
nice to use both since bATPase is highly expressed and ROC1 was fairly
lowly expressed.  Sorry I was so sketchy on the details, write me if you
want the specifics.
Mike
-- 
Michael Prigge                          Phone: (541) 346-4256
Institute of Molecular Biology            Fax: (541) 346-5891
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