Sonication....

[jqpublic at home.net]

Hi,

Anyone know of an online "Sonication Primer" to get me up to speed?
Specifically what I'm trying to learn is:

1. What are typical power settings(in watts, not %) and some typical
protocols for E. coli lysis.

2. Effects of volume of cell concentration(i.e. is a semi-saturated 1 mL
volume a good 'model' for 1 liter of saturated culture resuspended in 50
mL)

3. Effective(and quick) ways to determine the extent of cell lysis.

Thanks