Long shot...: internal PCR standard for melon

Mike Prigge prigge at darkwing.uoregon.edu
Thu Jun 24 19:55:27 EST 1999


In article <l03130300b395a1fe5c0a@[150.26.200.11]>,
torki at NOCS.TSUKUBA-NOC.AFFRC.GO.JP (moez torki) wrote:

> I'm interested in the same matter but to use it for melon PCR
> investigation. I know that *very* few of you may perform molecular research
> on this truck, but maybe somone has an idea of a conserved constituve gene
> in cucurbits or whathever that could help.
> Thanks,
> Moez
> 
I would get the tobacco mito. beta-ATPase sequence and blast search the
databases.  This gene is highly expressed (should have lots of ESTs) and
quite well conserved.  A labmate and I have used the Arabidopsis version
for RNAse protections and RT-PCR, respectively.  Check Kelly, et al. 1990
Plant Cell 2:963 for references regarding the tobacco homolog and its use
as a Northern control.
Mike
-- 
Michael Prigge                          Phone: (541) 346-4256
Institute of Molecular Biology            Fax: (541) 346-5891
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