Marking the position in cell culture dish
dykim at bioneer.kaist.ac.kr
Thu Jun 24 21:23:15 EST 1999
Hi, I have difficulties in finding a specific position in my cell culture dishes.
I've been cultureing the rat striatal neuronal cells and now I plan to immunostain
the cells with NF-kB antibody. The problem is I want to double-stain the cells with
NADH-d NBT. This stain was not compatible with immunostaining(it quenches the rhodamine
and FITC fluorescence).
So, I immunostained the cells with rhodamine labeled anti-NF-kB, photographed
the cells, and then staining the cells with NADPH+NBT. But I could not find the
same position I photographed. I heard some uses the "Camera lucida" skill(drawing
the cell position and find it afterwise) but it was too laborious for this situation.
Does anyone knows the way to notify a specific position on the cell culture dishes
after photography? Is there any company who sells microscopically labeled culture dishes?
Please e-mail me if you have any idea or information for solving this problem.
Please help me. Any suggestions or comments about this situation will be greatly
Ajou medical school Dooyeon Kim ......................
Pharmacology Lab. dykim at bioneer.kaist.ac.kr
More information about the Methods