RT-PCR and pH

Neurootje nospam at czzorlnm.azn.nl
Fri Jun 25 08:30:17 EST 1999


Can anybody tell me the optimal pH for the reverse transcription of RNA? I'm
using MMLV and the buffer supplied with has pH8.3 but someone told me I
should use a buffer with tris-base (10 mM tris-base, 50 mM KCl, 0,1%
gelatin).
Has someone tested the efficiency of RT in different reaction buffers?

Rudi





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