RNase H degradation question

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Sun Jun 27 05:01:05 EST 1999


In article <Pine.OSF.3.95.990625090829.12769A-
100000 at helix.mgh.harvard.edu>, Arul Jayaraman <jayarama at helix.mgh.harvar
d.edu> writes
>I have a question on RNase H degradation.  Does the enzyme specifically
>snip or cleave a RNA:DNA hybrid at the site of binding or does it
>recogonize such a hybrid and cleave/degrade it anyplace? In other words,
>if I have RNaseH activity, would the RNa be completely degraded or would
>it result in 2 smaller pieces? 

Nice question!



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