PC12 / puromycin
wchoi at MED.CHUNGBUK.AC.KR
Sun Jun 27 07:57:51 EST 1999
I am trying to select PC12 cells which were transfected with pRT-On
recombinant. pRT-On has puromycin resistant marker. I did a kill-curve
experiment with various concentration of puromycin and found that even
high conc. of puromycin could not decrease the survival of PC12 cells no
less than 40%.
The manufacture's manual recommanded that 0.5-2 ug/ml of puromycin is
enough to select and maintain the stable transfectant. I am using 3
ug/ml of puromycin and control PC12 cells (not transfected) survived
I want to hear those with similar experiences.
 Does PC12 cell line really resistant to puromycin?
 Is there any alternate chemicals for puromycin in this case?
Thank you in advance.
Woong Choi, M.D.
wchoi at med.chungbuk.ac.kr
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