DNA purification from gel

John R. McQuiston zje8 at cdc.gov
Mon Jun 28 09:31:17 EST 1999


We have used a combination protocol that uses the BioRad Matrix and Buffer, 
and the Promega Columns and protocol.  This works really well under 2 kb (95%) 
but drops off quickly after that.  e-mail me if you want the full procedure.

John 










In article <Pine.LNX.4.04.9906281149320.11933-100000 at m31-036.azn.nl>, 
maurice at m31-036.azn.nl says...
>
>Hello all
>
>I usually purify my DNA from gel using phenol freeze-fraction or
>freeze-squeeze methods. I don't like organic solvents however, so I am
>looking for a decent alternative for fragments <1kb. Anyone?
>Thanks for any help
>
>Maurice van Steensel
>
>Azh nazg durbatal^uk, azh nazg gimbatul,
>Azh nazg thrakatal^uk agh burzum ishi krimpatul!
>                -- J. R. R. Tolkien




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