DNA purification from gel

Hiranya S. Roychowdhury hroychow at NMSU.EDU
Mon Jun 28 11:58:19 EST 1999


At 11:50 AM 6/28/99 +0200, Maurice van Steensel wrote:
>Hello all
>
>I usually purify my DNA from gel using phenol freeze-fraction or
>freeze-squeeze methods. I don't like organic solvents however, so I am
>looking for a decent alternative for fragments <1kb. Anyone?
>Thanks for any help
>
>Maurice van Steensel
>
>Azh nazg durbatal^uk, azh nazg gimbatul,
>Azh nazg thrakatal^uk agh burzum ishi krimpatul!
>		-- J. R. R. Tolkien
>
>

Yes.
Just leave the phenol out in the freeze-squeeze method.


Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu




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