cloning large pieces of DNA

[joshi pradeep]

Hi Molbio enthusiasts!
I am having difficulty cloning a large fragment (>10kb) into pBS
(bluescript).
Any suggestions! ( with  regards to bugs, vectors, ligation  anything)
Thanks

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Pradeep M. Joshi
MOCB Program,					1031-#2,Maiden Choice Ln,
Dept. Of Biological Sciences,			Baltimore, MD-21229
1000, Hilltop Circle,
Baltimore, MD - 21250
Tel Office: 410-455-1334/3122			Res: 410-247-1744

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