GFP labeled plasma membrane marker

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Mon Jun 28 13:43:42 EST 1999


Christer Ericsson <ericsson at acsu.buffalo.edu> wrote:
> What I really would need for a marker would be a good "prototypical"
> GFP-labeled glycosylated integral membrane protein, which sorts to the
> plasma membrane efficiently enough to serve as a marker.

Stable cell lines with about any GPCR work just fine :-) Personally, I
use beta2-adrenergic receptor which has the disadvantage that it will
internalize upon addition of agonist, but there are other GFP-tagged
GPCRs out there which might be more trafficking-resistant.

OTOH, I remember reading an article in Biochemical Journal some time
ago where the plasma membrane was counterstained by trypan blue
fluorescence.  Is there any particular reason that you want to use a
fluorescent protein?

--Cornelius.

PS: Next time you want to post something in multiple newsgroups, please
    use crossposting instead of multiposting. Thank you.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */



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