Overcooked southern blots!
sweechie at HOTMAIL.COM
Tue Jun 29 03:58:35 EST 1999
To whom it may concern,
I made a big mistake in my southern blot in that I overcooked the
membrane at 80 degrees.Instead of the usual 2 hours or so,I left it in the
over for a good 7 hours.Is the membrane of no use or can it be saved
somehow.I desperately need sone feedback from anyone who may know what to do
in this case.PLEASE HELP!
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