Overcooked southern blots!
Frank O. Fackelmayer
Frank.Fackelmayer at uni-konstanz.de
Tue Jun 29 05:25:06 EST 1999
No problem at all. Baking at 80degrees is a method to couple the blotted DNA to
the membrane. It works, but I guess nobody really knows how. Anyway, I have
also sometimes left the membrane bake overnight, with no negative effect. So,
7h is clearly on the safe side.
Gayatri Mani wrote:
> To whom it may concern,
> I made a big mistake in my southern blot in that I overcooked the
> membrane at 80 degrees.Instead of the usual 2 hours or so,I left it in the
> over for a good 7 hours.Is the membrane of no use or can it be saved
> somehow.I desperately need sone feedback from anyone who may know what to do
> in this case.PLEASE HELP!
> Thanks all.
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