Fast Western Blots

Chi Ong cong8 at burnet.edu.au
Tue Jun 29 06:03:59 EST 1999


I am having trouble with the a fast Western blot protocol as provided by the
Nen Lifesciences PVDF membrane am using Amersham ECL reagants to detect
conjugated HRP. My problem is that the signal that I am getting is very
faint or sometimes absent.
What are peoples experiences with this with fast westerns, or does anyone
have a particular protocol that they would recommend. Any advice or comments
would be appreciated.
Thanks in Advance
    Chi





More information about the Methods mailing list