Boosting protein expression in mammalian cells ?
nicholas_theodorakis at urmc.rochester.edu
Tue Jun 29 10:59:20 EST 1999
In article <3778D9F0.8AAF332B at cajal.mbb.ki.se>,
svend at cajal.mbb.ki.se wrote:
> Dear All,
> Rumours around this lab have it that there is a drug out there, which
> may enhance the yield of transient heterologue protein expression in
> mammalian cells, e.g. CHO cells ?
> Can anyone confirm this and even better, name the compound ?
> Thanks for any input,
> Svend Kjaer, Ph.D.
> Dept. of Neuroscience,
> Karolinska Institute,
IIRC, butyrate enhances expression from some promoters (e.g., CMV
promoter) a few-fold in transfected cells. If nobody else remembers,
I'll try to come up with a reference for you.
| Nick Theodorakis |
| nicholas_theodorakis at urmc.rochester.edu |
| (previously theodorn at gusun.georgetown.edu) |
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