glycosylation in insect cells ?
svend at CAJAL.MBB.KI.SE
Tue Jun 29 12:44:12 EST 1999
I am expressing the extracellular portion of a human receptor in
baculovirus-infected insect cells as an Fc-fusion protein in order to
raise antibodies to the receptor part. So far, so good. I get dozens of
different antibodies using phage-antibody libraries.
However, few (or none) of these (and they are not Fc binders !) seem to
recognise the full-length receptor expressed in mammalian cells, which
leaves me a bit bewildered. Therefore, the question goes; is it possible
that the glycosylation machinery in insect cells glycosylates the
protein in a manner which is dramatically (or at least somewhat)
different from that observed in mammalian cells ?
If this is indeed the fact, the differing reactivity of the antibodies
can be explained by altered epitopes resulting from different
Any input appreciated,
Svend Kjaer, Ph.D.
Dept. of Neuroscience,
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