Inverse PCR

Nicolas von Ahsen nvahsen at hotmail.com
Tue Jun 29 13:31:34 EST 1999


Hi Axel,

must it be inverse PCR? Or try this:
P.D. Siebert, A. Chenchik, D. E. Kellogg, K. A. Lukyanov, and S. A.
Lukyanov. An improved PCR method for walking in uncloned genomic DNA.
Nucleic Acids Res 23:1087-1088, 1995.
It works. There is also a kit available from Clontech using this
method, but you can cook it yourself. It works.

Best regards
Nicolas




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