Urea and ion exchange

Ricardo Silva ricardo.silva at CIGB.EDU.CU
Tue Jun 29 18:56:43 EST 1999

Hi all,
Several colleagues of mine have advised me against using high 
concentrations (8 M) of urea in ion exchange runs. In their 
experience, nothing binds under this conditions. This puzzles me, 
because being urea an uncharged species, it shouldn't interfere with 
ion exchange, right?
Does anybody have similar experiences and/or have an explanation?


Alejandro Martin
Division of Vaccines,
PO Box 6162
La Habana, Cuba
CP 10600

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