danger from uv light in BSC?
deborah.hailstones at smtpgwy.agric.nsw.gov.au
Tue Jun 29 19:14:34 EST 1999
I'm very interested to hear other peoples' thoughts and experiences
about occupational health and safety issues associated with using the
uv light in a BSC.
I have a colleague who is getting increasingly heated about the use of
the uv light (5-10 minutes) before using a BSC to add template to
diagnostic PCR. I like to do this to cross link (ie attempt to
'remove') any exogenous template which may be present, since the
cabinet is used by many people for many things, including the
inoculation of microorganisms. I like to think that this procedure
reduces the chance of cross-contaminating my reactions (though I
realise opinions obviously differ on this).
My colleague, however, is convinced that this presents a serious
hazard in terms of a) uv radiation (not my use of the word) leaking
from within the cabinet (which is sealed using the shield provided)
and b) the action of the light on the air inside, which is producing
free radicals "including ozone".
I agree that direct exposure to excess uv is a concern (and that
nobody should enter the cabinet whilst the uv is turned on! ;-) ), but
does anyone out there have any evidence/opinion on this matter? I'm
all for OH&S, so I'm interested to see what the general feeling is.
I know that this type of question often leads to many spinoffs, so can
reiterate that I am asking *specifically* about the effects/dangers of
uv light, in a covered BSC, on other workers in the room whilst the
light is on and then when the shield is removed and the BSC turned on.
Thanks in advance for any contribution,
More information about the Methods