cloning large pieces of DNA

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Tue Jun 29 02:56:41 EST 1999


In article <Pine.SGI.3.96A.990628152341.1995390A-100000 at umbc7.umbc.edu>,
joshi pradeep <joshi at umbc.edu> writes
>I am having difficulty cloning a large fragment (>10kb) into pBS
>(bluescript).
>Any suggestions! ( with  regards to bugs, vectors, ligation  anything)
>Thanks

Try a lower copy vector just in case the E.coli doesn't like what you
are cloning. i.e. pBR322, pACYC etc.

Duncan 
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk



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