cloning large pieces of DNA

Dr. Duncan Clark Duncan at
Tue Jun 29 02:56:41 EST 1999

In article <Pine.SGI.3.96A.990628152341.1995390A-100000 at>,
joshi pradeep <joshi at> writes
>I am having difficulty cloning a large fragment (>10kb) into pBS
>Any suggestions! ( with  regards to bugs, vectors, ligation  anything)

Try a lower copy vector just in case the E.coli doesn't like what you
are cloning. i.e. pBR322, pACYC etc.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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