cloning large pieces of DNA
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Tue Jun 29 02:56:41 EST 1999
In article <Pine.SGI.3.96A.990628152341.1995390A-100000 at umbc7.umbc.edu>,
joshi pradeep <joshi at umbc.edu> writes
>I am having difficulty cloning a large fragment (>10kb) into pBS
>Any suggestions! ( with regards to bugs, vectors, ligation anything)
Try a lower copy vector just in case the E.coli doesn't like what you
are cloning. i.e. pBR322, pACYC etc.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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