2k in an exon? that's the coding part. if the insert is in frame and w/o stop codons, it should work OK or do you mean intron (the part that's spliced out) ? then it depends on the type of splice site and the position of the insert good luck, Rogier, MicFizz, Free U of A **** Posted from RemarQ - http://www.remarq.com - Discussions Start Here (tm) ****