small DNA purification

Mark Bowen mb at laplace.csb.yale.edu
Wed Jun 30 08:25:12 EST 1999


Does anyone have any useful tips for the purification of dsDNA less than
80bp from enzymatic reactions?  I've tried Qiagen products and Bio101
but both give very low recovery.  I currently use phenol:chloroform
which works but it is very time consuming.

--
Mark Bowen
HHMI/Yale University
Dept. of Molecular Biophysics and Biochemistry





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