Alkaline phosphatase blocking

paulam8610 at paulam8610 at
Wed Jun 30 10:29:39 EST 1999

 I am using a plaque lift technique and would like to block alkaline
phosphatase produced by E. coli which is growing on the agar. I am using
a secondary antibody alkaline phosphatase conjugate and need this
conjugated AP to be the only one detected. I have had great difficulty
finding specific information on this and would appreciate any help on
locating a relevant source.

P. Murphy

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