Alkaline phosphatase blocking

Peter Ashby p.r.ashby at
Wed Jun 30 11:31:02 EST 1999

In article <7ldd4h$h86$1 at>, paulam8610 at wrote:

> Hi,
>  I am using a plaque lift technique and would like to block alkaline
> phosphatase produced by E. coli which is growing on the agar. I am using
> a secondary antibody alkaline phosphatase conjugate and need this
> conjugated AP to be the only one detected. I have had great difficulty
> finding specific information on this and would appreciate any help on
> locating a relevant source.
I con't comment specifically on bacteria but for AP detection in embryos
you block background by heating to 65C and/or incubation with Levamisole
@2mM (sigma). 
Hope this helps.


Peter Ashby
Wellcome Trust Building
University of Dundee
Dundee, Scotland
Reverse the spam and remove to email me.

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