Alkaline phosphatase blocking
p.r.ashby at dundee.MAPS.ac.uk
Wed Jun 30 11:31:02 EST 1999
In article <7ldd4h$h86$1 at nnrp1.deja.com>, paulam8610 at my-deja.com wrote:
> I am using a plaque lift technique and would like to block alkaline
> phosphatase produced by E. coli which is growing on the agar. I am using
> a secondary antibody alkaline phosphatase conjugate and need this
> conjugated AP to be the only one detected. I have had great difficulty
> finding specific information on this and would appreciate any help on
> locating a relevant source.
I con't comment specifically on bacteria but for AP detection in embryos
you block background by heating to 65C and/or incubation with Levamisole
Hope this helps.
Wellcome Trust Building
University of Dundee
Reverse the spam and remove to email me.
More information about the Methods