epitope duplication

Bernard P. Murray, PhD bpmurray*STUFFER* at socrates.ucsf.edu
Wed Jun 30 18:10:20 EST 1999

In article <930738157.14195 at www.remarq.com>, rogier
<stuger at cellbiology.uni-frankfurt.de> wrote:

> Hi,
> how big is your epitope?
> If it is small enough, just stick some oligos in your
> plasmid.
> If it's too big for oligos, PCR it out with overhanging
> primers for IDENTICAL restriction sites,

Or, if you want to ensure the tandem inserts are all in the same
orientation, use primers with different restriction sites but
with cohesive ends (eg. BamHI/BglII) then you can cut the
mix with these enzymes to eliminate any products containing
head-to-head copies.


Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA

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