Urea and ion exchange

Bernard P. Murray, PhD bpmurray*STUFFER* at socrates.ucsf.edu
Wed Jun 30 18:13:52 EST 1999

In article <B0001705182 at spider.cigb.edu.cu>, ricardo.silva at cigb.edu.cu wrote:

> Hi all,
> Several colleagues of mine have advised me against using high 
> concentrations (8 M) of urea in ion exchange runs. In their 
> experience, nothing binds under this conditions.

Did they deionise their urea before use?  With this concentration
any trace contaminants could be significant.  Some commercial
grades of urea are quite dirty.


Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA

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