Urea and ion exchange

Bernard P. Murray, PhD bpmurray*STUFFER* at socrates.ucsf.edu
Wed Jun 30 18:13:52 EST 1999


In article <B0001705182 at spider.cigb.edu.cu>, ricardo.silva at cigb.edu.cu wrote:

> Hi all,
> Several colleagues of mine have advised me against using high 
> concentrations (8 M) of urea in ion exchange runs. In their 
> experience, nothing binds under this conditions.

Did they deionise their urea before use?  With this concentration
any trace contaminants could be significant.  Some commercial
grades of urea are quite dirty.

   Bernard

-- 
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA



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