Methods June 1999 Archives by thread
Starting: Tue Jun 1 03:21:47 EST 1999
Ending: Wed Jun 30 23:06:06 EST 1999
Messages: 666
- DNA extraction from recalcitrant tissues
Gonzalo
- desalting DNA
C.S. Wilding
- Problems with Southerns
roger morton
- Help! The yield of my large-scale plasmid DNA is low!
Wolfgang Schechinger
- wrong orientation of ligated fragment
Gregor Kopitar
- Basic Subcloning Question
Chris Boyd
- Best life science vendor Web site?
Joan Evans
- desalting DNA
Hiranya S. Roychowdhury
- wrong orientation of ligated fragment
Hiranya S. Roychowdhury
- GROWTH in minimal MEDIA (M9)
F. Ferreira
- apoptosis...
INNO007
- ADV: Web site improvement
sales at NS1.SUBMISSIONSERVICE.COM
- competent bacteria
Chris Kafer
- RT-PCR troubleshooting
Sarah Roberts-Thomson
- synthetic E coli promoter?
John Dixon
- wrong orientation of ligated fragment
Yuji Tai
- DNA-Isolation from human hair
Gav
- Phage contaminatio
Marcus Wernitz
- Problems with Southerns!
Gayatri Mani
- (none)
zxn
- Supplier of Elutip-d columns?
Mathieu Ingouff
- tom.weaver@incyte.com
Dr. Duncan Clark
- molecular beacons
Thomas Burmeister
- Plant biotechnology club
Fiers-Vercruysse
- male-specific phage
MOUNIR IZALLALEN
- definition of "leaky promoter"
Peter
- Beta-galactosidase as reporter
Mimi Liao
- DIG footprinting
John Lum
- RT-PCR and Negative Controls&Contamination
ddam at uh.edu
- PolyA signal database
kacrejam
- Light cycler
Raja Kota
- Maleic Acid
Mismatch 420
- 2-mercaptoethanol and DTT
Mismatch 420
- desalting DNA
Hiranya S. Roychowdhury
- drug targeting....
R. Jayakumar
- cleavage of CD18
lebowski13 at yahoo.com
- DNA synthesis of arbitrary sequences
Zasha Weinberg
- RNA Pellet viscosity (Pectic acid?)
Jørgen Borrebæk
- Sigma chitinase qualitative assay
Stephan Heeb
- in situ hybridization for oil palm flowers
Dr.Sh.Shahrul Rabiah
- pGEM-T vectors
Antony Halsall
- agarose electrophoresis tank @ $10
Dr. Mohd Razip Samian
- probe stripping
Halina Sobolewska
- sAV-magnet. particles/biot. DNA & NH3
Carsten Hohoff
- diverse genomic DNAs
soenke behrends
- Plasmid DNA spont. digesting - the long version :-)
plxsrt at pln1.nott.ac.uk
- in situ hybridization request
Aude Lamy
- phage display systems
AlPiEs
- Cell attachment assay
Sebastien Paris
- in vitro TX with chromatin template DNA
Fab
- probe stripping
David L. Haviland, Ph.D.
- Antennapedia
SHAW John
- Strand-specific RT-PCR
Johan Burger
- Baculovirus Titer Kit--ADVICE NEEDED
mccubbinv at omrf.ouhsc.edu
- PE Biosystems MicroSeq Kit
"MALLARY, LAURA L. ", JSC-SD
- Damn those chickens!
bbeitzel at biomail.ucsd.edu
- what is the meaning of TT measure?
.Foti Marcelo
- what is the meaning of TT measure?
.Foti Marcelo
- Basic Subcloning Question
David L. Haviland, Ph.D.
- 2000-Search Engines
submititzlll at MAILEXCITE.COM
- Southern problems... too small of a probe?
Ray Lara
- Southern problems... too small of a probe?
Ray Lara
- NESTED PCR of a nested PCR, necessary?
Ray Lara
- how to isolate a large plasmid
root at titus.u-strasbg.fr
- A survey, one-step western-blot
immunechem
- tissue preservative buffers
joe cook
- how to isolate a large plasmid
root at titus.u-strasbg.fr
- mRNA isolation directly from tissue or cultured cells
O. de Leeuw
- Online discount lab supplies
D. Jacobson
- PCR Troubleshooting References
stewartl at alkami.com
- 5000 Free Sites!! (99654)
box20037871 at aol.com
- unsubscribe
jie lv
- in situ hybridization request
Aude Lamy
- strand specific RT-PCR
Johan Burger
- Stable mRNA's?
Jørgen Borrebæk
- Detecting RNA
Jørgen Borrebæk
- Peptide sequence selection software
WZhao68
- Does Ethidium bromide retard RNA migration?
Mark Garry Petten
- pCS2+ vector sequence
Dean G. Stathakis
- separation of cardiac endosomes from plasma membrane
Will Fuller
- when is an essential gene essential
DrD
- seeking the antibody to FSH and LH of rats
xiangli87 at yahoo.com
- seeking the antibody to FSH and LH of rats
xiangli87 at yahoo.com
- Precipitating viruses without ultracentrifuge??
Dr Mark Watson
- AFLP bands
Raja Kota
- Recombinant DNA Techniques Workshop
Mark Cauchi
- ssDNA and dsDNA after asymetric PCR
juamber at my-deja.com
- Want to buy a PCR-Machine
Dirk Junghans
- Stable Transfection of T Cells
Alan J Watson
- 10X LB?
Shiao Wang
- Transgenics/database/program
Larry Fliegel
- myocardial actin Antibody querry
theodosios73 at my-deja.com
- About VR1012 Vical's vector
amherrera at CIGB.EDU.CU
- ELISA for MuLV
guy lemay
- ABI 377 Genotyping Standards
Dr. Paul N. Hengen
- single stranded DNA prep
Malathi Hari
- PCR Primer Design Tools Review
stewartl at alkami.com
- 'Cracking'
Dr. Robert Gay
- unsubscribe
jie lv
- who is working with strep-tag vectors??
Peter
- antibody specific only for osteoblasts and antibody specific only for osteoclasts
Dr. Andreas Schubert
- unsubscribe
Dr ranajit k ghosh
- phage DNA
Adrian Clarke
- Anyone tried the pIZ/V5-His vector for expression in insect cells?
Frank O. Fackelmayer
- Goat anti mouse Cy2 or ALEXA TM 488 labelled antibodies
joe
- ABI Genotyping Standards
Robert Chadwick
- pH electrodes for bio samples
D. Jacobson
- nucleotide sequence retrieval
Theodosios Kyriakou
- (none)
wangkun
- on ELFO buffer
.Foti Marcelo
- nucleotide sequence retrieval
inderpal singh
- Exact buffer calculations
Cecil Jack
- PCR and Genomic DNA contamination
Dandog
- Open debate: agarose inhibition :-/
moez torki
- Corex Tubes???
Scott Currie
- Open debate: agarose inhibition :-/
moez torki
- cRACE problem
Don Walthers
- 'Cracking'
S.Nakayama, NIAR
- does a method exist for direct automated sequencing of a RAPD fragment
Arthur
- gel retardation assay
Inge Depoortere
- test
Inge Depoortere
- Sensitivity of polyclonals on Western
Dmitry Belostotsky
- poly A mRNA in bacteria?
"", David Alexander
- prtoteinase digestion
inderpal singh
- AFLP problems
inderpal singh
- Want to buy a PCR-Machine
Hiranya S. Roychowdhury
- DNA extraction from amnioti cells
Nicola Cucurachi
- Corex Tubes???
Hiranya S. Roychowdhury
- Promega's GeneEditor
Denis LeBel
- ADV: ANALYSTS CHOOSE "ADLU" MICROCAP BESTOCK PICK
stocks99 at USA.NET
- Owl Semi-Dry Transfer Apparatus
Connie Runyan
- ADV: ANALYSTS CHOOSE "ADLU" MICROCAP BESTOCK PICK
stocks99 at USA.NET
- ADV: ANALYSTS CHOOSE "ADLU" MICROCAP BESTOCK PICK
stocks99 at USA.NET
- protein MW
Jun Zhang
- RNA polymerase ability
Neal Robert Melvin
- MicroArray on Glass
Michael Megginson
- Corex Tubes???
tfitzwater at NEXSTAR.COM
- toxic protein
junz at sfu.ca
- About plasmid's restriction endonuclease digest
zxn
- unsubscribe
jie lv
- Problems with SacI
Martin Offterdinger
- pMALC2 with His-tag?
flathmann.horst at mh-hannover.de
- Poly(A) addition signals?
Ian A. York
- Multichannel combs
John R. McQuiston
- source for info about specific proteins
Tim Bloom
- protein MW
Hiranya S. Roychowdhury
- Vista Green
Dr. Kris Partridge
- Gene Expression Database Parameters
Harry Mangalam
- genome mismatch scan enzymes source?
RPrestonic
- Glycerol PAGE "toughening"
Daniel Kim
- Recommendations for cheaper "stratacooler" benchtop coolers?
Chris H Lindley
- GCG to MSWORD
Jeremy Murray
- GCG to MSWORD
David L. Haviland, Ph.D.
- FS Unused Bio-Rad Pulsed Field Power Module
TonyS2
- request: mouse mAB against mouse tissue??
Frank Dehner
- David
Andrei Ulitin
- Yeast cDNA library
Frank O. Fackelmayer
- Cell lysis buffer/reagents
Russell, Rachael
- Cell lysis buffer/reagents
Russell, Rachael
- Regular power supply for electroporation?
ciufo at zdna.micro.umass.edu
- Fluorescent probe labelled dTTP
Dr Antony Halsall
- Help
zhaojun
- Isotope labelled GPP
Dr. Shan Lu
- (none)
Dr. Shan Lu
- Protocol for GFP compatible BrdU staining?
Christian Zuppinger
- geneclean - better recovery of gDNA?
C.S. Wilding
- MW of a protein.
Mr. G. Morley
- "Re: wrong orientation of ligated fragment"
Narayanan Karthikeyan
- screening for DNA-binding proteins
Andy Cannons
- dam- E.coli
Timothy P. Beary
- Recommendations for cheaper "stratacooler" benchtop coolers?
tfitzwater at NEXSTAR.COM
- Regular power supply for electroporation?
tfitzwater at NEXSTAR.COM
- Parameters for Southern Hybs!
Gayatri Mani
- GFP nd BrdU detection incompatibilies
Christian Zuppinger
- pGEM-T vectors
R. Jayakumar
- restriction enzymes
ML
- QIAGEN native lysis protocol
Kevin Urbanic
- Histological and Histopathological Evaluation of the Testis
Bernhard Herrmann
- prtoteinase digestion
.Krimer Alejandro
- prtoteinase digestion
.Krimer Alejandro
- Cell doubling times
L.D.
- Corex Tubes
Dr. JE Knapp
- RV: bacterial expression systems
.Krimer Alejandro
- See For Yourself
Cindy
- In vivo Apoptosis detection by laser?
Missy Tuck
- RACE systems/ TRIzol and RNAWiz
Jill Johnston
- Help
Elisabeth Liu
- Quantitative PCR fine tuning
Robert Passey
- Needed: Used T-cycler; T-illuminator; etc.
j.ongerth at unsw.edu.au
- source of dNTPs for PCR
alex dobrovic
- Cell doubling times
Joe Chandler
- Cell doubling times
Jean-Philippe Nougayrède
- GFP and quantitation
Stewart P Johnson
- (none)
Dr. Shan Lu
- Cloning nomenclature
Bill_A_Nussbaumer at ms.bd.com
- D-18mm sonicater tip
Aki Hoji
- pUC19 Blue/White riddle.
Phillip San Miguel
- (none)
Hiranya S. Roychowdhury
- pBridge
Ralf Weigel
- Protocols
Pierre Rodrigues
- G418/Geneticin
Edward Dunphy
- screening library/microarrays?
Amy Avery
- Human Bax
Younes Bounhar
- Glycerol PAGE "toughening"
David L. Haviland, Ph.D.
- Problem using pfuturbo for PCR
Alan Smith
- Stable transfections....
Dr. Robert Gay
- Trehalulose
Simon Hansom
- SDS-PAGE and guanidine....
jqpublic at home.net
- Problem using pfuturbo for PCR
moez torki
- HER2/neu ECD Expression in CHO cells
Gregory P. Adams, Ph.D.
- Best substrate for Western-HRP
Martin Offterdinger
- Best substrate for Western-HRP
WSchick at AOL.COM
- Cathepsin L inhibitor supplier
none
- Source of ToxB ?
Jean-Philippe Nougayrède
- JOBOP RESEARCH ASSOCIATE MONOCLONAL ANTIBODIES
915608 at candseek.com
- DNA quantification
Kevin Urbanic
- flag or hA-tag?
Jonas
- In answer to: Removing GuHCl prior to PAGE
Jonas
- JOBOP SCIENTIST IN-VIVO ANGIOGENESIS
915608 at candseek.com
- DNA quantification
Bill_A_Nussbaumer at ms.bd.com
- S. pombe
Harutyan Melkonyan
- Sequencing Gel Boxes.
Marie Estock
- Nonradioactive DNA end-labelling
Pranvera Ikonomi
- JOBOP Scientist / Glucose Assay
915603 at candseek.com
- reuse of Qiaquick columns
Frederik Boernke
- Best substrate for Western-HRP
Paul S. Brookes.
- Mp3 FREE Site www.mediterranei.it/mp3
maxim
- Mp3 FREE Site www.mediterranei.it/mp3
no.email.address.entered at none444.yet
- [Q] 18% SDS-PAGE
Namjin Chung
- Asking for DRs Birch, Gatehouse and Shinozaki
ma
- Recommendation please for good red/green secondary antiboy combo
S. Findley
- help
abing_w at 263.NET
- hi..plasmid curing
R. Jayakumar
- PCR Cloning
m_ravi_gupta at yahoo.com
- phiX174
Dr. Duncan Clark
- Assay for lipid/fatty acid binding proteins
Kev
- for pPIC9 restriction endonuclease
zxn
- DNA end labelling
Pranvera Ikonomi
- Northern hybridisation
Martin Dalziel
- SDS-PAGE and Guanidinium
Jonas
- Northern hybridisation
David L. Haviland, Ph.D.
- Do you want to hear Italian Progressive Rock in MP3 for FREE? Go on www.mp3.com/greyowl
nobody at nowhere33.yet
- on free nuclease water
.Foti Marcelo
- on free nuclease water
.Foti Marcelo
- iCycler from Bio-Rad
Dr. Dietrich Mäde
- help
abing_w at 263.NET
- (none)
zxn
- Mediterranei On Line: Video, Mp3, folklore of sicilians people.
nobody at nowhere33.yet
- ASSAY FOR S-ADENOSYL-L-HOMOCYSTEINE HYDROLASE
Dr Vinayak Kanade
- ASSAY FOR S-ADENOSYL-L-HOMOCYSTEINE HYDROLASE
Dr Vinayak Kanade
- ASSAY FOR S-ADENOSYL-L-HOMOCYSTEINE HYDROLASE
Dr Vinayak Kanade
- ASSAY FOR S-ADENOSYL-L-HOMOCYSTEINE HYDROLASE
Santosh N. Pawar
- ASSAY FOR S-ADENOSYL-L-HOMOCYSTEINE HYDROLASE
Santosh Pawar
- Review any book, PC or mac software pgm, consumer products and keep them!
lindabredenberg at BIGFOOT.COM
- help
huiyang
- help
huiyang
- help
abing_w at 263.NET
- Micro sample pH probe
Adam Benson
- Books about molecular biology?
klaus_frommerr at my-deja.com
- transfection
Albrecht Hoffmeister
- internal standard for RT-PCR in plant
myoon at hort.cri.nz
- Test
JIO Paul
- Long PCR Primer Behavior
Michael E. Zwick
- Shortcuts to molecular biology protocols and method forum
Long
- Correction to: Shortcuts to molecular biology protocols and method forum
Smbp
- MBS news!
Pierre Rodrigues
- Bacterial conjugation
Paul Beare
- Death of Primary Dopaminergic neuron culture.
Chen Linan
- Search for pT7.7 sequence
One Night
- site directed mutagenesis
Fiers-Vercruysse
- Pharmacia PlusOne Silverstrain kit
Rene Nederhand
- ? on protein extraction
Ruben Zamora
- glutathione-sepharose storage
Ian Mc
- 96 Well DNA ELISA?
John R. McQuiston
- Anyone using gene chips?
Robert M. Mihalek
- GCG to MSWORD
Bill_A_Nussbaumer at ms.bd.com
- Electroporation of embryos and explants
Dirk Junghans
- Explant and embryo electroporation
Dirk Junghans
- imidazole and degradation
Eva Horak
- Y2H library
biosci-request at net.bio.net
- Long shot...: internal PCR standard for melon
moez torki
- help
beisz at SUN5.IBP.AC.CN
- RNA gel elution possible?
Karthik Aghoram
- ccd-camera & software
Hannes Klump
- ? Spontaneous precip of alphahelix protein
tob at ssi.dk
- Primers for sequencing from EMBL4?
Allen Gathman
- Needed: Vector for C-terminal GST fusion expression in E. coli.
Tim Szeto
- Lung sections for influenza
Rick Bright
- Need a *cheap* recirculating bath..suggestions?
Jil Tardiff
- Methicillin revelation
Phillip San Miguel
- Cuvettes
inderpal singh
- (none)
Rosie Redfield
- Oops, subject is Autoclave purchase advice
Rosie Redfield
- Autoclave purchase advice
Rosie Redfield
- Cloning vector needed: Eco-Not-Bgl2 sites
S. Findley
- Aphidicolin
Mimi Liao
- Help please on Genomic DNA extraction of CHO cells.
Mad-Woof
- immunolocolization vs IHC?
JLove98905
- help
beisz at SUN5.IBP.AC.CN
- Auto-antibodies in mouse, how frequent?
Troels Wind
- BIOSCI/bionet miniFAQ & Fundraiser
BIOSCI Administrator
- Southern hybridization
Dr.Sh.Shahrul Rabiah
- tyrosine phosphorylation
Robert Geisler
- tyrosine phosphorylation
Robert Geisler
- Southern hybridization
Hiranya S. Roychowdhury
- TRAP-Assay
Dr. Holger Olbrueck
- Sonication....
jqpublic at home.net
- Marking the position in cell culture dish
Dooyeon Kim
- Great protocol site
Long
- (none)
zoubende at 263.NET
- coimmunoprecipitation
David Humair
- Hypoxia model for cultured cells
Adriani Kanellopoulos
- cDNA Screening service
Klaus Lun
- genotype of hyperpermeable E. coli AS19
Rick L
- RNase H degradation question
Arul Jayaraman
- RT-PCR and pH
Neurootje
- Search for pTrc99A plasmid.
Christophe Jacob
- cDNA from blood - protocol
Soenke Behrends
- Arul
Andrei Ulitin
- 'Humanized' luciferase
TJ Murphy
- Psychic Net comment
TJ Murphy
- PC12 / puromycin
Woong Choi
- Immunodepletion?
Shun-Chang Wang
- New Site about East Timor
DrCursor
- DNA purification from gel
Maurice van Steensel
- mycotoxins
ivo naydenov
- Measure micro dissolved O2 with pH meter or PC
Bob Olson
- GFP labeled plasma membrane marker
Christer Ericsson
- DNA purification from gel
Hiranya S. Roychowdhury
- on a program to draw plasmid.
.Foti Marcelo
- Southern hybridization
inderpal singh
- Acid Washing Cuvettes
mbarnhart at my-deja.com
- Refolding His-tagged proteins
Peter S. Galatin
- cloning large pieces of DNA
joshi pradeep
- iCycler
Dr. Dietrich Mäde
- hemoglobin
Dr. Dietrich Mäde
- ADV: Make a killing from home in the Travel industry.
travels44 at USA.NET
- Bio Rad Data View
Chris Upton
- Conferences in US/Europe
Raja Kota
- Conferences in US/Europe
Raja Kota
- Once In A Lifetime...
Hal
- reuse of centricons?
Thomas O Westerling
- Overcooked southern blots!
Gayatri Mani
- Fast Western Blots
Chi Ong
- bGal reporter Q
Mark Bowen
- Boosting protein expression in mammalian cells ?
Svend Kjaer
- Inverse PCR
Axel Schwekendiek, Ph.D.
- Boosting protein expression in mammalian cells ?
David L. Haviland, Ph.D.
- receptor binding
Caroline Szymeczek-Seay
- glycosylation in insect cells ?
Svend Kjaer
- Bubbles in DNA melting Tm analysis
Antonio Jiménez Ruiz
- RNease Qiagen Kit HELP!
Jeff Huckaby
- one stupid question
fxl4 at HUNNY.INS.CWRU.EDU
- glycosylation in insect cells ?
DOCTORHIM at AOL.COM
- latex gloves and ethidiumbromide
Alexander Brands
- internal translation termination
blight
- epitope duplication
Ashu Sharma
- epitope duplication
Ashu Sharma
- Save 40-60% on Cellular & Digital Phone Accessories
icwpcs at aol.com
- Any comments from Visible Genetics OpenGene system users?
John Barlow
- Measuring pH of Tris solutions
Ricardo Silva
- Urea and ion exchange
Ricardo Silva
- danger from uv light in BSC?
Deborah Hailstones
- a stupid question
fxl4 at PO.CWRU.EDU
- A Complete Chemistry Measurement System For Monitoring Cell Culture Media
bioanalyzer at hotmail.com
- submitting a paper to TTO?
alex dobrovic
- protein expression in EL-4 stable transfectants
Soo Jung Yoon
- LambdaGem-11 cloning problems
Susanne Rafelski
- sexual PCR
zxn
- Urea and ion exchange
.Krimer Alejandro
- Reverse Northern Dot Blots
Microbiology
- Reverse Northern Dot Blot
Microbiology
- small DNA purification
Mark Bowen
- Question concerning GST
Jeffrey Shane Temple
- (no subject)
Eduard Post
- problems with taq-polymerase
Eduard Post
- DNA labelling/detection kits ?
Andrew Sharp
- Alkaline phosphatase blocking
paulam8610 at my-deja.com
- Voted #1 e-business
ebusiness2000 at HEMEROTECA.ICFES.GOV.CO
- New Blasticidin Selection
Paul Jasper
Last message date:
Wed Jun 30 23:06:06 EST 1999
Archived on: Sun Mar 6 21:59:54 EST 2005
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