yeast extraction using PIERCE Y-PER

Christian Fankhauser cfankhauser at AIM.SALK.EDU
Mon Mar 1 17:44:36 EST 1999


Hello everyone,

extracting proteins out of yeast with glass beads and hard shacking can be
tricky, Pierce now offers an alternative with their Y-PER Yeast Protein
Extraction Reagent, the problem is that it cost 200$ for 500ml. I know that
it contains phosphate buffer, high salt and some ionic detergent but does
anyone know the exact composition? or have a reference for this? or have
any experience with it?

Thanks for your suggestions

Christian Fankhauser

Christian Fankhauser
PBIO
The Salk Institute
10010 North Torrey Pines road
La Jolla CA-92037   USA
phone (619) 453 4100 X 1129
FAX (619) 558 6379





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