TRANFECTION?

amherrera at CIGB.EDU.CU amherrera at CIGB.EDU.CU
Mon Mar 1 13:11:31 EST 1999


Hi all,
I read in articles that to select a stable transfectants with antibiotic 
G418 you need a concentration of 600-800 micrograms/ml and to 
maintain the transfectants a concentration of 200 micrograms/ml is 
needed.
My question is when do I have to change the antibiotic     
concentration ? or do I have to stay working at the high 
concentration until appears colonies of cell?, by the way I'm 
working with multiplying cells.
Thanks a lot.
Eddy Ernesto




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