I read in articles that to select a stable transfectants with antibiotic
G418 you need a concentration of 600-800 micrograms/ml and to
maintain the transfectants a concentration of 200 micrograms/ml is
My question is when do I have to change the antibiotic
concentration ? or do I have to stay working at the high
concentration until appears colonies of cell?, by the way I'm
working with multiplying cells.
Thanks a lot.