RT-PCR buffer help

Georg Kroeger a03m at biologie.uni-bremen.de
Tue Mar 2 02:29:27 EST 1999


Hi,

we are going to screen  l o t s  of samples in RT-PCR and therfore
would like to do one-tube-reactions (like the boehringer Titan-Kit).
Unfortunately we are unable to reproduce the high sensivity of
boehringer's lovely (but for our porposes unaffordable) kit. It looks
like our reaction buffer is missing the magic agent X as our homemade
enzyme mix works well in b's buffer.
Who could give us a working  recipe for a one-tube buffer? Thanks in
advance!

Georg



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