we are going to screen l o t s of samples in RT-PCR and therfore
would like to do one-tube-reactions (like the boehringer Titan-Kit).
Unfortunately we are unable to reproduce the high sensivity of
boehringer's lovely (but for our porposes unaffordable) kit. It looks
like our reaction buffer is missing the magic agent X as our homemade
enzyme mix works well in b's buffer.
Who could give us a working recipe for a one-tube buffer? Thanks in