BAC DNA prep for end sequencing

Nancy Phillips, M.D. phillinj at SLU.EDU
Mon Mar 1 22:55:04 EST 1999


Hello, does anyone have a favorite miniprep protocol for BAC and PAC 
sequencing-quality DNA? So far, the alkaline lysis method is not working 
for me, and I suspect there is some finesse beyond that required for 
ordinary-sized plasmids. Thanks for any help. 

Nancy Phillips
phillinj at slu.edu
Department of Pathology
St. Louis University Hospital
3635 Vista Ave.
St. Louis, MO, 63110, USA
ph:(314)577-8782
fax:(314)268-5120

ph



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