BAC DNA prep for end sequencing

Nancy Phillips, M.D. phillinj at SLU.EDU
Mon Mar 1 22:55:04 EST 1999

Hello, does anyone have a favorite miniprep protocol for BAC and PAC 
sequencing-quality DNA? So far, the alkaline lysis method is not working 
for me, and I suspect there is some finesse beyond that required for 
ordinary-sized plasmids. Thanks for any help. 

Nancy Phillips
phillinj at slu.edu
Department of Pathology
St. Louis University Hospital
3635 Vista Ave.
St. Louis, MO, 63110, USA


More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net