RNA isolation

Tom Belbin tbelbin at morgan.ucs.mun.ca
Mon Mar 1 09:10:51 EST 1999


In article <7auu00$clk$1 at pulp.ucs.ualberta.ca>, Rick Lau
<lmll at gpu.srv.ualberta.ca> wrote:

> Hi, 
> 
> There was a posting about the one-step method for isolation of total RNA
> from all kinds of cells(G+, G- and mammalian...) in this group, but I
> couldn't find it by any searching. Can anybody post it again, or tell me
> where is the original source? 
> 
> Thanks
> 
> Rick
> (To email me, please change 'll' to '11')

Rick,

Are you referring to the use of guanidinium thiocyanate with phenol and
chloroform to prepare RNA?

See Chomczynski and Sacchi (1987) Analytical Biochemistry 162: 156-159.

Hope this helps,

Tom Belbin
Biochemistry Department
Memorial University of Newfoundland




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