Best his-tag and myc-tag antibodies?

Rich Dudley rdudley+ at pitt.edu
Tue Mar 2 08:54:32 EST 1999


"D. Pearton" wrote:

> Hi all,
>
> I'm soliciting opinions as to the best source of his-tag and myc-tag
> antibodies.  I've had problems detecting my his-tag fusion protein with
> the invitrogen antibodies and I was wondering which companies you've had
> the most success with.
>
> Thanks in advance,
> Yak

Dave--

I like Qiagen's Hexa-his antibody.  They also have a penta-his, which in my
experience gives a little better signal, but higher background (meaning in
this case spurrious bands).  The hexa-his had a lower signal, but was more
specific.

Good luck!

rich

--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
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