RT-PCR buffer help
John R. McQuiston
zje8 at cdc.gov
Tue Mar 2 07:19:39 EST 1999
I would reccommend Amersham/Pharmacia's (No Aff.) Ready to go RT-PCR beads.
They cost the same as a PCR mix and they are very consistant. You may be able
to work out a deal if you need alot of them. You can also get them in 96 well
format I think.
In article <36db8f9c.589717 at news.zfn.uni-bremen.de>,
a03m at biologie.uni-bremen.de says...
>we are going to screen l o t s of samples in RT-PCR and therfore
>would like to do one-tube-reactions (like the boehringer Titan-Kit).
>Unfortunately we are unable to reproduce the high sensivity of
>boehringer's lovely (but for our porposes unaffordable) kit. It looks
>like our reaction buffer is missing the magic agent X as our homemade
>enzyme mix works well in b's buffer.
>Who could give us a working recipe for a one-tube buffer? Thanks in
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