caetano at dilbert.ucdavis.edu
Tue Mar 2 16:45:49 EST 1999
I am trying to use DOP_PCR to amplify genomic DNA obtained microdissected
chromosomes. My ultimate goal is to create a chromosome-specific
library with the DOP-PCR products for isolating microsatellite
sequences. My first question: does anyone have experience with this?
A colleague from another university has tried this but was able to
isolate very few microsatellites from her library. Have any of you tried
this before? I would appreciate any comments or ideas.
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