IUBio

Complementation

Malay curiouser at ccmb.ap.nic.in
Tue Mar 2 12:58:53 EST 1999


I am posting the message again hoping for a reply

Malay
curiouser at ccmb.ap.nic.in

-----Original Message-----
From: Malay <curiouser at ccmb.ap.nic.in>
To: bionet.molbio.methds-reagnts mail newsgroup <bionet-news at dl.ac.uk>
Date: Monday, February 22, 1999 10:42 PM
Subject: Complementation


>Dear Netters:
>
>I am trying to complement the strain UQ285 of E.coli, carrying a
temperature
>sensitive mutation of sigma-70 gene with a genomic library of a Pseudomonas
>strain. I transform and plated several dilutions of the transformed cells.
>When I plate around 400-500 cells per plate I get nothing. But when I plate
>almost a lawn of cells I get some colonies growing but very poorly.
>
>My question is as follows:
>
>Does the density of the plating has anything to do with the complemation of
>a temperature sensitive strain? If so then what is the ideal plating titre?
>
>Any comments or suggestion will be appreciated.
>
>Malay K Basu
>curiouser at ccmb.ap.nic.in
>
>
>
>
>
>
>




More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net