IUBio

DOP-PCR

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Wed Mar 3 04:16:22 EST 1999


In article <7bhm6d$84a$1 at mark.ucdavis.edu>, Alexandre Caetano
<caetano at dilbert.ucdavis.edu> writes
>I am trying to use DOP_PCR to amplify genomic DNA obtained microdissected
>chromosomes.  My ultimate goal is to create a chromosome-specific
>library with the DOP-PCR products for isolating microsatellite
>sequences.  My first question:  does anyone have experience with this?
>A colleague from another university has tried this but was able to
>isolate very few microsatellites from her library.  Have any of you tried
>this before?  I would appreciate any comments or ideas.
>
>Thank you
>
>alex
>

I saw a paper yesterday on getting this working in a very recent NAR i.e
No's 1-4 of this year. Can't remember which one. 

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk



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