DIG High Prime

Gerd gerdn at ibg.uit.no
Wed Mar 3 04:22:05 EST 1999


John F Mackay etc wrote:
> 
> Hi Josh,
> 
> Make sure you DNA is phenol/chloroform purified...no other way!  Variability
> can be due to variable gel extractions etc. whereas phenol/chloroform will
> clean it up.

We rutinely use Qiagen gel extraction; no problem at all.
Phenol/Chloroform is a huge mess,  takes much longer time, and in my
hands do not give better results.

Gerd



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