John F Mackay etc wrote:
>> Hi Josh,
>> Make sure you DNA is phenol/chloroform purified...no other way! Variability
> can be due to variable gel extractions etc. whereas phenol/chloroform will
> clean it up.
We rutinely use Qiagen gel extraction; no problem at all.
Phenol/Chloroform is a huge mess, takes much longer time, and in my
hands do not give better results.