DNA molarity

Anke Wichert anke.wichert at charite.de
Wed Mar 3 10:10:52 EST 1999


Hi Antony,

to calculate the molarity, you have to know the exact length of your DNA
- best was, if you also knew how many of each nucleotide, but mostly the
length is enough. Now, Ausubel et al. give the nucleotide molar masses
in their "Current protocols" as well as "Short protocols" (for the
latter: p A2-9). The masses of the dNTPs are 491,2 (dATP), 467,2 (dCTP),
507,2 (dGTP) - dTTP is not given. But remember, that in DNA you have got
dNMPs, so the masses you have to calculate with are 331,2 (dAMP), 307,2
(dCMP), and 347,2 (dGMP). Normally, I just multiply the number of bases
with 309 g/mol x base, if necessary, double the result for dsDNA and
have got the molar mass in g/mol.

Hope this helped - Anke Wichert
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