>To: methods at net.bio.net>From: a03m at biologie.uni-bremen.de (Georg Kroeger)
>Subject: coprecipitant for RNA
>Date: Wed, 03 Mar 1999 07:53:31 GMT
>Reply-To: a03m at biologie.uni-bremen.de>NNTP-Posting-Host: bioanalytik-2.uft.uni-bremen.de
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>>We have problems to precipitate small amounts of RNA (invisible
>pellets got lost sometimes). Any hint for a nice coprecipitant would
>be appreciated. By the way, at which point will I get rid of the
>precipitant (70% EtOH whash?) again?
>Thanks for your help!
Depends on what you want to do with the RNA. For most things, 10µg of
yeast tRNA makes a nice big pellet. Where other RNAs might interfere,
I prefer glycogen. I have also tried linear polyacrylamide, ala a
recipe I found here around two years ago. It also works well, but
requires an effort to make it up. None of these will be removed in
the wash as far as I know, so you pretty much have to chose a carrier
that won't interfere with downstream applications.
brett at borcim.wustl.edu
Dept of Molecular Microbiology
Washington University School of Medicine