unexpected-size transcript

jerryzhou at 126.com jerryzhou at 126.com
Wed Mar 3 08:20:07 EST 1999


In article <7bhj4p$f44$1 at nnrp1.dejanews.com>,
  mbarnhart at my-dejanews.com wrote:
> Jerry,
>
> Can you give a little more detail as to how you made the transgenic plant?
> IE, what vector did you use to get your gene of interest into the protoplast?
>  That might help the group come up with some ideas.
>
> Michael Barnhart
> Laboratory Supervisory
> Connective Tissue Physiology Lab
> University of Houston
>
> In article <7bh0hg$tvm$1 at nnrp1.dejanews.com>,
>   jerryzhou at 126.com wrote:
> >      when I detect the transcription of a foreign gene in a transgenic rice
> >      plants by northern blotting, I got several signal bands rather
> >      larger than the expected size. I can exclude the DNA contamination
> >      will you please explain this?
> >
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>
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the vector I used for my transgenic rice plant contains actin1-geneA-nos and
a hpt cassette.the probe i used is the cDNA of geneA labelled by random
priming. I can also exclude the unspecific hybridization of 25s and 18s RNA
signal. Could you explain this? BTW, I use bombardment for gene transfer.

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