IUBio

EDTA in growth media

Chen Ho An chen at bsm.bioc.ucl.ac.uk
Wed Mar 3 16:22:20 EST 1999


I've seen a couple of recipes of minimal media with EDTA added, but can't
work out why it's there.  Does anyone knows the reason?  It occurs to me
that perhaps EDTA serves a reservoir of divalent cations which can be
released when needed, but surely EDTA has a higher affinity for the cations
than enzymes, how else could it inactive enzymes?







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