"berg" <b.j.vanbergenhenegouwen at pharm.uu.nl> wrote:
>Hi there, I have a question.
>>Does somebody have a WORKING protocol for mammalian expression cloning of
>cDNA libraries into COS or other mammalian cells?
>>if you do, which vector and which COS (or other cellsystem) did you use and
>what was the result?
>>Or maybe can you direct me to a company which sell kits for this kind of
Invitrogen sells cDNA libraries (supposedly full length, checked a
couple of 5' ends - were fine) in SV40-ori containing vector. This
should work in Cos cells if you do not plan LONG (>6 d) selection.
Otherwise, run-away replication of plasmids will kill the cell.
Stratagene - as far as I recall - has libraries in a vector with EBV
ori-P, which should not have these problems. However, you should find
a cell line which expresses EBNA to support the replicastion
(actually, if you DO find such human cells, I will appreciate a
Several other companies were rumored to have similar libraries.
No affiliation with any of the above....
Victor Levenson MD/PhD
Res Asst Prof
UIC, Dept of Genetics M/C 669
900 S Ashland Ave The one who wants finds HOW
Chicago IL 60607 The one who doesn't finds WHY
levenson at uic.edu