I have used Gibco TRIzol reagent several times to purify total RNA from tissue
culture. It was convenient to use, the price was reasonable , and I got the
desired product by subsequent RT-PCR using the total RNA directly.
rashie athukorala wrote:
> can anyone suggest a protocol or reasonably priced kit that might work. I
> plan on doing RT-PCR on the RNA.
Graduate Research Assistant
Department of Biochemistry
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Tel (765) 494-6591 Fax (765) 494-7897
Email: jeong at biochem.purdue.edu