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protocol for total RNA purification from placental tissue

Jee-Yeong Jeong jeong at biochem.purdue.edu
Thu Mar 4 13:20:40 EST 1999


I have used Gibco TRIzol reagent several times to purify total RNA from tissue
culture. It was convenient to use, the price was reasonable , and I got the
desired product by subsequent RT-PCR using the total RNA directly.

rashie athukorala wrote:

> can anyone suggest a protocol or reasonably priced kit that might work.  I
> plan on doing RT-PCR on the RNA.
>
> thanks.

--
===================================================
Jee-Yeong Jeong
Graduate Research Assistant
Department of Biochemistry
Purdue University
Hansen Life Science Research Building, Room 225
West Lafayette, IN 47907
Tel (765) 494-6591          Fax (765) 494-7897
Email: jeong at biochem.purdue.edu





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