The best way is to equilibrate and run the separation with the same
concentration of guanidine or urea.
I have worked with several proteins without any problems,
Hope this helps.
> -----Mensaje original-----
> De: ross_turbyfill at wrsmtp-ccmail.army.mil> [SMTP:ross_turbyfill at wrsmtp-ccmail.army.mil]
> Enviado el: Wednesday, March 03, 1999 10:40 AM
> Para: methods at net.bio.net> Asunto: FPLC under Denaturing Conditions
>> Does anyone have a protcol for performing FPLC separations (size
> under denaturing conditions, i.e., in the presence of guanidine HCl, urea,
> DTT, etc? Specifically, are the concentrations of the buffers used in the
> chromatography the same concentrations used to denature the proteins
> 8M Urea or 6M GuHCl)?
>> Any advice anyone has to offer would be greatly appreciated!!
>>ross_turbyfill at NOSPAMwrsmtp-ccmail.army.mil>>>> -**** Posted from remarQ, Discussions Start Here(tm) ****-
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